Differential growth of wrinkled biofilms

Biofilms are antibiotic-resistant bacterial aggregates that grow on moist surfaces and can trigger hospital-acquired infections. They provide a classical example in biology where the dynamics of cellular communities may be observed and studied. Gene expression regulates cell division and differentiation, which affect the biofilm architecture. Mechanical and chemical processes shape the resulting structure. We gain insight into the interplay between cellular and mechanical processes during biofilm development on air-agar interfaces by means of a hybrid model. Cellular behavior is governed by stochastic rules informed by a cascade of concentration fields for nutrients, waste and autoinducers. Cellular differentiation and death alter the structure and the mechanical properties of the biofilm, which is deformed according to Foppl-Von Karman equations informed by cellular processes and the interaction with the substratum. Stiffness gradients due to growth and swelling produce wrinkle branching. We are able to reproduce wrinkled structures often formed by biofilms on air-agar interfaces, as well as spatial distributions of differentiated cells commonly observed with B. subtilis.Comment: 19 pages, 13 figure

Intrinsic viscosity of a suspension of weakly Brownian ellipsoids in shear

We analyze the angular dynamics of triaxial ellipsoids in a shear flow subject to weak thermal noise. By numerically integrating an overdamped angular Langevin equation, we find the steady angular probability distribution for a range of triaxial particle shapes. From this distribution we compute the intrinsic viscosity of a dilute suspension of triaxial particles. We determine how the viscosity depends on particle shape in the limit of weak thermal noise. While the deterministic angular dynamics depends very sensitively on particle shape, we find that the shape dependence of the intrinsic viscosity is weaker, in general, and that suspensions of rod-like particles are the most sensitive to breaking of axisymmetry. The intrinsic viscosity of a dilute suspension of triaxial particles is smaller than that of a suspension of axisymmetric particles with the same volume, and the same ratio of major to minor axis lengths.Comment: 14 pages, 6 figures, 1 table, revised versio

Settling of an asymmetric dumbbell in a quiescent fluid

We compute the hydrodynamic torque on a dumbbell (two spheres linked by a massless rigid rod) settling in a quiescent fluid at small but finite Reynolds number. The spheres have the same mass densities but different sizes. When the sizes are quite different the dumbbell settles vertically, aligned with the direction of gravity, the largest sphere first. But when the size difference is sufficiently small then its steady-state angle is determined by a competition between the size difference and the Reynolds number. When the sizes of the spheres are exactly equal then fluid inertia causes the dumbbell to settle in a horizontal orientation.Comment: 11 pages, 1 figure, as publishe

Effect of weak fluid inertia upon Jeffery orbits

We consider the rotation of small neutrally buoyant axisymmetric particles in a viscous steady shear flow. When inertial effects are negligible the problem exhibits infinitely many periodic solutions, the "Jeffery orbits". We compute how inertial effects lift their degeneracy by perturbatively solving the coupled particle-flow equations. We obtain an equation of motion valid at small shear Reynolds numbers, for spheroidal particles with arbitrary aspect ratios. We analyse how the linear stability of the \lq log-rolling\rq{} orbit depends on particle shape and find it to be unstable for prolate spheroids. This resolves a puzzle in the interpretation of direct numerical simulations of the problem. In general both unsteady and non-linear terms in the Navier-Stokes equations are important.Comment: 5 pages, 2 figure

Dynamics of bacterial aggregates in microflows

Depto. de Análisis Matemático y Matemática AplicadaFac. de Ciencias MatemáticasFALSEunpu